Kim JJ, Chan PPY, Vlassakis J, Geldert A, Herr AE. Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells. Small. 2018 Nov;14(48):e1802865. doi: 10.1002/smll.201802865. Epub 2018 Oct 17.
Time:
Nov 06,2023
Kim JJ, Chan PPY, Vlassakis J, Geldert A, Herr AE. Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells. Small. 2018 Nov;14(48):e1802865. doi: 10.1002/smll.201802865. Epub 2018 Oct 17. PMID: 30334351; PMCID: PMC6272123.
為了在單細胞蛋白質(zhì)印跡中結(jié)合蛋白質(zhì)標記物，使用微孔分離單個細胞和蛋白質(zhì)標記物包被的微粒。磁場將蛋白質(zhì)包被的微粒引導(dǎo)至>75%的微孔，以便 1）將蛋白質(zhì)標記物的量子傳遞到每個充滿細胞的微孔，2）使蛋白質(zhì)標記物的增溶與細胞裂解同步。
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